Cryo- EM breaks 2 Ångström Barrier

14/06/2018

Atomic Resolution Cryo-EM Structure of β-Galactosidase, Bartesaghi et al.

Cryo-electron microscopy (EM) approaches enable determination of structures of a variety of protein complexes at near-atomic resolution. These advances help to determine a cryo-EM density map for β-galactosidase bound to the inhibitor phenylethyl β-D-thiogalactopyranoside where the ordered regions are resolved at a level of detail seen in X-ray maps at ∼ 1.5 Å resolution. Using this density map in conjunction with constrained molecular dynamics simulations provides a measure of the local flexibility of the non-covalently bound inhibitor and offers further opportunities for structure-guided inhibitor design.