GROUP KöHLER GOTTFRIED | Biochemistry & Biophysics, Structural & Computational Biology
Biomolecular optical spectroscopy
Dr. Bohr-Gasse 9 (VBC 5), 1030 Vienna | Room: 1.616
Consecutive threading of cyclodextrin macrocycles on green fluorescent coumarin dye was studied using fluorescence correlation spectroscopy. Inclusion complexes of up to three macrocycles could be resolved. This construct becomes an ideal water-soluble stain for lipid structures in live cell imaging.
Biophysical characterisation of biomolecules and of their interactions in solution as well as on a live cell level represents the main object of our research. Methods include fluorescence and time resolved techniques performed over a wide range of time resolution. Studies by optical spectroscopy are complemented by biocalorimetry (DSC).
Quantitative studies on molecular dynamics on a single molecule level are performed using advanced fluorescence correlation techniques. Among others, these methods are applied on studies of ligand-receptor interactions relevant for hormone regulation and...