Publication archive till 2003

Bacher, G., Szymanski, W. W., Kaufman, S. L., Zollner, P., Blaas, D., and Allmaier, G. (2001). Charge-reduced nano electrospray ionization combined with differential mobility analysis of peptides, proteins, glycoproteins, noncovalent protein complexes and viruses, J Mass Spectrom 36, 1038-52.


Bayer, N., Prchla, E., Schwab, M., Blaas, D., and Fuchs, R. (1999). Human rhinovirus HRV14 uncoats from early endosomes in the presence of bafilomycin, FEBS Lett 463, 175-8.

Bayer, N., Schober, D., Huttinger, M., Blaas, D., and Fuchs, R. (2001). Inhibition of clathrin-dependent endocytosis has multiple effects on human rhinovirus serotype 2 cell entry, J Biol Chem 276, 3952-62.

Bayer, N., Schober, D., Prchla, E., Murphy, R. F., Blaas, D., and Fuchs, R. (1998). Effect of bafilomycin A1 and nocodazole on endocytic transport in HeLa cells: implications for viral uncoating and infection, J Virol 72, 9645-55.

Benie, A. J., Moser, R., Bauml, E., Blaas, D., and Peters, T. (2003). Virus-ligand interactions: identification and characterization of ligand binding by NMR spectroscopy, J Am Chem Soc 125, 14-5.

Blaas, D., Kuechler, E., Vriend, G., Arnold, E., Luo, M., and Rossmann, M. G. (1987). Comparison of the three-dimensional structure of two human rhinoviruses (HRV2 and HRV14), Proteins 2, 263-72.

Blaas, D., Patzelt, E., and Kuechler, E. (1982). Cap-recognizing protein of influenza virus, Virology 116, 339-48.

Blaas, D., Patzelt, E., and Kuechler, E. (1982). Identification of the cap binding protein of influenza virus, Nucleic Acids Res 10, 4803-12.

Blaas, D., Rucheton, M., and Jeanteur, P. (1977). [Evidence of protein kinase activity in 2 murine oncornaviruses], C R Acad Sci Hebd Seances Acad Sci D 285, 269-72.

Blaas, D., Rucheton, M., and Jeanteur, P. (1979). Characterization and properties of a protein kinase associated with murine sarcoma-leukemia virus (MSV-MuLV). Endogenous phosphorylation of a unique p10 polypeptide, Biochem Biophys Res Commun 87, 272-80.

Blom, N., Hansen, J., Blaas, D., and Brunak, S. (1996). Cleavage site analysis in picornaviral polyproteins: discovering cellular targets by neural networks, Protein Sci 5, 2203-16.

Brabec, M., Baravalle, G., Blaas, D., and Fuchs, R. (2003). Conformational changes, plasma membrane penetration, and infection by human rhinovirus type 2: role of receptors and low pH, J Virol 77, 5370-7.


Chavan, A. J., Rychlik, W., Blaas, D., Kuechler, E., Watt, D. S., and Rhoads, R. E. (1990). Phenyl azide substituted and benzophenone-substituted phosphonamides of 7-methylguanosine 5'-triphosphate as photoaffinity probes for protein synthesis initiation factor eIF-4E and a proteolytic fragment containing the cap-binding site, Biochemistry 29, 5521-9.


Duechler, M., Ketter, S., Skern, T., Kuechler, E., and Blaas, D. (1993). Rhinoviral receptor discrimination: mutational changes in the canyon regions of human rhinovirus types 2 and 14 indicate a different site of interaction, J Gen Virol 74 ( Pt 10), 2287-91.

Duechler, M., Skern, T., Blaas, D., Berger, B., Sommergruber, W., and Kuechler, E. (1989). Human rhinovirus serotype 2: in vitro synthesis of an infectious RNA, Virology 168, 159-61.

Duechler, M., Skern, T., Sommergruber, W., Neubauer, C., Gruendler, P., Fogy, I., Blaas, D., and Kuechler, E. (1987). Evolutionary relationships within the human rhinovirus genus: comparison of serotypes 89, 2, and 14, Proc Natl Acad Sci U S A 84, 2605-9.

Gruenberger, M., Pevear, D., Diana, G. D., Kuechler, E., and Blaas, D. (1991). Stabilization of human rhinovirus serotype 2 against pH-induced conformational change by antiviral compounds, J Gen Virol 72 ( Pt 2), 431-3.

Gruenberger, M., Wandl, R., Nimpf, J., Hiesberger, T., Schneider, W. J., Kuechler, E., and Blaas, D. (1995). Avian homologs of the mammalian low-density lipoprotein receptor family bind minor receptor group human rhinovirus, J Virol 69, 7244-7.

Guarne, A., Kirchweger, R., Verdaguer, N., Liebig, H. D., Blaas, D., Skern, T., and Fita, I. (1996). Crystallization and preliminary X-ray diffraction studies of the Lb proteinase from foot-and-mouth disease virus, Protein Sci 5, 1931-3.


Hewat, E., and Blaas, D. (2001). Structural studies on antibody interacting with viruses, Curr Top Microbiol Immunol 260, 29-44.

Hewat, E. A., and Blaas, D. (1996). Structure of a neutralizing antibody bound bivalently to human rhinovirus 2, Embo J 15, 1515-23.


Hewat, E. A., Marlovits, T. C., and Blaas, D. (1998). Structure of a neutralizing antibody bound monovalently to human rhinovirus 2, J Virol 72, 4396-402.

Hewat, E. A., Neumann, E., and Blaas, D. (2002). The concerted conformational changes during human rhinovirus 2 uncoating, Mol Cell 10, 317-26.

Hewat, E. A., Neumann, E., Conway, J. F., Moser, R., Ronacher, B., Marlovits, T. C., and Blaas, D. (2000). The cellular receptor to human rhinovirus 2 binds around the 5-fold axis and not in the canyon: a structural view, Embo J 19, 6317-25.

Hofer, F., Berger, B., Gruenberger, M., Machat, H., Dernick, R., Tessmer, U., Kuechler, E., and Blaas, D. (1992). Shedding of a rhinovirus minor group binding protein: evidence for a Ca(2+)-dependent process, J Gen Virol 73 ( Pt 3), 627-32.

Huber, M., Brabec, M., Bayer, N., Blaas, D., and Fuchs, R. (2001). Elevated endosomal pH in HeLa cells overexpressing mutant dynamin can affect infection by pH-sensitive viruses, Traffic 2, 727-36.


Kienberger, F., Stroh, C., Kada, G., Moser, R., Baumgartner, W., Pastushenko, V., Rankl, C., Schmidt, U., Muller, H., Orlova, E., et al. (2003). Dynamic force microscopy imaging of native membranes, Ultramicroscopy 97, 229-37.


Kirchweger, R., Ziegler, E., Lamphear, B. J., Waters, D., Liebig, H. D., Sommergruber, W., Sobrino, F., Hohenadl, C., Blaas, D., Rhoads, R. E., and et al. (1994). Foot-and-mouth disease virus leader proteinase: purification of the Lb form and determination of its cleavage site on eIF-4 gamma, J Virol 68, 5677-84.


Kovar, H., Jug, G., Auer, H., Skern, T., and Blaas, D. (1991). Two dimensional single-strand conformation polymorphism analysis: a useful tool for the detection of mutations in long DNA fragments, Nucleic Acids Res 19, 3507-10.

Kowalski, H., Maurer-Fogy, I., Vriend, G., Casari, G., Beyer, A., and Blaas, D. (1989). Trypsin sensitivity of several human rhinovirus serotypes in their low pH-induced conformation, Virology 171, 611-4.

Kowalski, H., Maurer-Fogy, I., Zorn, M., Mischak, H., Kuechler, E., and Blaas, D. (1987). Cleavage site between VP1 and P2A of human rhinovirus is different in serotypes 2 and 14, J Gen Virol 68 ( Pt 12), 3197-200.



Kronenberger, P., Schober, D., Prchla, E., Blaas, D., and Fuchs, R. (1997). Use of free-flow electrophoresis for the analysis of cellular uptake of picornaviruses, Electrophoresis 18, 2531-6.

Kronenberger, P., Schober, D., Prchla, E., Ofori-Anyinam, O., Vrijsen, R., Rombaut, B., Blaas, D., Fuchs, R., and Boeye, A. (1998). Uptake of poliovirus into the endosomal system of HeLa cells, Arch Virol 143, 1417-24.

Liebig, H. D., Skern, T., Luderer, M., Sommergruber, W., Blaas, D., and Kuechler, E. (1991). Proteinase trapping: screening for viral proteinase mutants by alpha complementation, Proc Natl Acad Sci U S A 88, 5979-83.

Liebig, H. D., Ziegler, E., Yan, R., Hartmuth, K., Klump, H., Kowalski, H., Blaas, D., Sommergruber, W., Frasel, L., Lamphear, B., and et al. (1993). Purification of two picornaviral 2A proteinases: interaction with eIF-4 gamma and influence on in vitro translation, Biochemistry 32, 7581-8.

Luderer, M., Liebig, H. D., Sommergruber, W., Blaas, D., Kuechler, E., and Skern, T. (1992). Using proteinase trapping to detect revertants of inactive rhinoviral 2A proteinase mutants, Biol Chem Hoppe Seyler 373, 523-8.

Luderer, M. E., Hofer, F., Hagspiel, K., Allmaier, G., Blaas, D., and Kubicek, C. P. (1991). A re-appraisal of multiplicity of endoglucanase I from Trichoderma reesei using monoclonal antibodies and plasma desorption mass spectrometry, Biochim Biophys Acta 1076, 427-34.

Marlovits, T. C., Abrahamsberg, C., and Blaas, D. (1998). Soluble LDL minireceptors. Minimal structure requirements for recognition of minor group human rhinovirus, J Biol Chem 273, 33835-40.

Marlovits, T. C., Abrahamsberg, C., and Blaas, D. (1998). Very-low-density lipoprotein receptor fragment shed from HeLa cells inhibits human rhinovirus infection, J Virol 72, 10246-50.

Marlovits, T. C., Zechmeister, T., Gruenberger, M., Ronacher, B., Schwihla, H., and Blaas, D. (1998). Recombinant soluble low density lipoprotein receptor fragment inhibits minor group rhinovirus infection in vitro, Faseb J 12, 695-703.

Marlovits, T. C., Zechmeister, T., Schwihla, H., Ronacher, B., and Blaas, D. (1998). Recombinant soluble low-density lipoprotein receptor fragment inhibits common cold infection, J Mol Recognit 11, 49-51.

Mischak, H., Kolch, W., Hofer, F., Weissinger, E., Gessl, A., Davidson, W. F., Aiello, F. B., Blaas, D., and Rapp, U. R. (1990). A raf/myc virus immortalized macrophage cell line which supports the growth of B-cell and B-cell hybridomas, Oncogene 5, 1377-82.

Mischak, H., Neubauer, C., Berger, B., Kuechler, E., and Blaas, D. (1988). Detection of the human rhinovirus minor group receptor on renaturing Western blots, J Gen Virol 69 ( Pt 10), 2653-6.

Mischak, H., Neubauer, C., Kuechler, E., and Blaas, D. (1988). Characteristics of the minor group receptor of human rhinoviruses, Virology 163, 19-25.

Morand, M., Blaas, D., and Kenndler, E. (1997). Reduction of wall adsorption in capillary zone electrophoresis of a basic single-chain antibody fragment by a cationic polymeric buffer additive, J Chromatogr B Biomed Sci Appl 691, 192-6.


Neubauer, C., Frasel, L., Kuechler, E., and Blaas, D. (1987). Mechanism of entry of human rhinovirus 2 into HeLa cells, Virology 158, 255-8.

Neumann, E., Moser, R., Snyers, L., Blaas, D., and Hewat, E. A. (2003). A cellular receptor of human rhinovirus type 2, the very-low-density lipoprotein receptor, binds to two neighboring proteins of the viral capsid, J Virol 77, 8504-11.


Okun, V., Ronacher, B., Blaas, D., and Kenndler, E. (2000). Capillary electrophoresis with postcolumn infectivity assay for the analysis of different serotypes of human rhinovirus (common cold virus), Anal Chem 72, 2553-8.

Okun, V. M., Blaas, D., and Kenndler, E. (1999). Separation and biospecific identification of subviral particles of human rhinovirus serotype 2 by capillary zone electrophoresis, Anal Chem 71, 4480-5.

Okun, V. M., Moser, R., Blaas, D., and Kenndler, E. (2001). Complexes between monoclonal antibodies and receptor fragments with a common cold virus: determination of stoichiometry by capillary electrophoresis, Anal Chem 73, 3900-6.

Okun, V. M., Moser, R., Ronacher, B., Kenndler, E., and Blaas, D. (2001). VLDL receptor fragments of different lengths bind to human rhinovirus HRV2 with different stoichiometry. An analysis of virus-receptor complexes by capillary electrophoresis, J Biol Chem 276, 1057-62.

Okun, V. M., Nizet, S., Blaas, D., and Kenndler, E. (2002). Kinetics of thermal denaturation of human rhinoviruses in the presence of anti-viral capsid binders analyzed by capillary electrophoresis, Electrophoresis 23, 896-902.

Okun, V. M., Ronacher, B., Blaas, D., and Kenndler, E. (1999). Analysis of common cold virus (human rhinovirus serotype 2) by capillary zone electrophoresis: the problem of peak identification, Anal Chem 71, 2028-32.

Okun, V. M., Ronacher, B., Blaas, D., and Kenndler, E. (2000). Affinity capillary electrophoresis for the assessment of complex formation between viruses and monoclonal antibodies, Anal Chem 72, 4634-9.

Patzelt, E., Blaas, D., and Kuechler, E. (1983). CAP binding proteins associated with the nucleus, Nucleic Acids Res 11, 5821-35.

Patzelt, E., Thalmann, E., Hartmuth, K., Blaas, D., and Kuechler, E. (1987). Assembly of pre-mRNA splicing complex is cap dependent, Nucleic Acids Res 15, 1387-99.

Penn, C. R., Blaas, D., Kuechler, E., and Mahy, B. W. (1982). Identification of the cap-binding protein of two strains of influenza A/FPV, J Gen Virol 62 (Pt 1), 177-80.

Pfistermueller, D. M., Blaas, D., and Hodits, R. A. (1996). Preferential recognition of the very low-density lipoprotein receptor ligand binding site by antibodies from phage display libraries, FEBS Lett 396, 14-20.

Prchla, E., Kuechler, E., Blaas, D., and Fuchs, R. (1994). Uncoating of human rhinovirus serotype 2 from late endosomes, J Virol 68, 3713-23.

Prchla, E., Plank, C., Wagner, E., Blaas, D., and Fuchs, R. (1995). Virus-mediated release of endosomal content in vitro: different behavior of adenovirus and rhinovirus serotype 2, J Cell Biol 131, 111-23.

Reischl, A., Reithmayer, M., Winsauer, G., Moser, R., Gosler, I., and Blaas, D. (2001). Viral evolution toward change in receptor usage: adaptation of a major group human rhinovirus to grow in ICAM-1-negative cells, J Virol 75, 9312-9.

Reithmayer, M., Reischl, A., Snyers, L., and Blaas, D. (2002). Species-specific receptor recognition by a minor-group human rhinovirus (HRV): HRV serotype 1A distinguishes between the murine and the human low-density lipoprotein receptor, J Virol 76, 6957-65.

Ronacher, B., Marlovits, T. C., Moser, R., and Blaas, D. (2000). Expression and folding of human very-low-density lipoprotein receptor fragments: neutralization capacity toward human rhinovirus HRV2, Virology 278, 541-50.

Rucheton, M., Blaas, D., and Jeanteur, P. (1978). Use of trypsin for rapid and efficient purification of murine sarcoma and leukemia virus, Biochimie 60, 1333-7.

Schnabel, U., Groiss, F., Blaas, D., and Kenndler, E. (1996). Determination of the pI of human rhinovirus serotype 2 by capillary isoelectric focusing, Anal Chem 68, 4300-3.

Schober, D., Kronenberger, P., Prchla, E., Blaas, D., and Fuchs, R. (1998). Major and minor receptor group human rhinoviruses penetrate from endosomes by different mechanisms, J Virol 72, 1354-64.

Skern, T., Duechler, M., Sommergruber, W., Blaas, D., and Kuechler, E. (1987). The molecular biology of human rhinoviruses, Biochem Soc Symp 53, 63-73.

Skern, T., Neubauer, C., Frasel, L., Grundler, P., Sommergruber, W., Zorn, M., Kuechler, E., and Blaas, D. (1987). A neutralizing epitope on human rhinovirus type 2 includes amino acid residues between 153 and 164 of virus capsid protein VP2, J Gen Virol 68 ( Pt 2), 315-23.

Skern, T., Sommergruber, W., Auer, H., Volkmann, P., Zorn, M., Liebig, H. D., Fessl, F., Blaas, D., and Kuechler, E. (1991). Substrate requirements of a human rhinoviral 2A proteinase, Virology 181, 46-54.

Skern, T., Sommergruber, W., Blaas, D., Gruendler, P., Fraundorfer, F., Pieler, C., Fogy, I., and Kuechler, E. (1985). Human rhinovirus 2: complete nucleotide sequence and proteolytic processing signals in the capsid protein region, Nucleic Acids Res 13, 2111-26.

Skern, T., Sommergruber, W., Blaas, D., Pieler, C., and Kuechler, E. (1984). Relationship of human rhinovirus strain 2 and poliovirus as indicated by comparison of the polymerase gene regions, Virology 136, 125-32.

Skern, T., Torgersen, H., Auer, H., Kuechler, E., and Blaas, D. (1991). Human rhinovirus mutants resistant to low pH, Virology 183, 757-63.

Skern, T., Zorn, M., Blaas, D., Kuechler, E., and Sommergruber, W. (1990). Protease or protease inhibitor?, Nature 344, 26.

Snyers, L., Zwickl, H., and Blaas, D. (2003). Human rhinovirus type 2 is internalized by clathrin-mediated endocytosis, J Virol 77, 5360-9.

Sommergruber, W., Ahorn, H., Klump, H., Seipelt, J., Zoephel, A., Fessl, F., Krystek, E., Blaas, D., Kuechler, E., Liebig, H. D., and et al. (1994). 2A proteinases of coxsackie- and rhinovirus cleave peptides derived from eIF-4 gamma via a common recognition motif, Virology 198, 741-5.

Sommergruber, W., Ahorn, H., Zophel, A., Maurer-Fogy, I., Fessl, F., Schnorrenberg, G., Liebig, H. D., Blaas, D., Kuechler, E., and Skern, T. (1992). Cleavage specificity on synthetic peptide substrates of human rhinovirus 2 proteinase 2A, J Biol Chem 267, 22639-44.

Sommergruber, W., Zorn, M., Blaas, D., Fessl, F., Volkmann, P., Maurer-Fogy, I., Pallai, P., Merluzzi, V., Matteo, M., Skern, T., and et al. (1989). Polypeptide 2A of human rhinovirus type 2: identification as a protease and characterization by mutational analysis, Virology 169, 68-77.

Thalmann, E., and Blaas, D. (1991). Synthesis of gamma-[4-(benzoylphenyl)methylamido]-7-methylguanosine 5'-triphosphate, a photoaffinity-label for cap-binding proteins, Biochim Biophys Acta 1088, 301-4.

Thalmann, E., Blaas, D., and Kuechler, E. (1990). Affinity chromatography of HeLa splicing extracts on m7 GTP-sepharose and photoaffinity-labeling of cap-binding proteins with gamma-[(4-benzoylphenyl) methylamido]-7-methyl-guanosine-5'-triphosphate, Mol Biol Rep 14, 207.

Torgersen, H., Blaas, D., and Skern, T. (1989). Low cost apparatus for primer-directed DNA amplification using Thermus aquaticus-DNA polymerase, Anal Biochem 176, 33-5.

Torgersen, H., Skern, T., and Blaas, D. (1989). Typing of human rhinoviruses based on sequence variations in the 5' non-coding region, J Gen Virol 70 ( Pt 11), 3111-6.

Tormo, J., Blaas, D., Parry, N. R., Rowlands, D., Stuart, D., and Fita, I. (1994). Crystal structure of a human rhinovirus neutralizing antibody complexed with a peptide derived from viral capsid protein VP2, Embo J 13, 2247-56.

Tormo, J., Centeno, N. B., Fontana, E., Bubendorfer, T., Fita, I., and Blaas, D. (1995). Docking of a human rhinovirus neutralizing antibody onto the viral capsid, Proteins 23, 491-501.

Tormo, J., Fita, I., Kanzler, O., and Blaas, D. (1990). Crystallization and preliminary x-ray diffraction studies of the Fab fragment of a neutralizing monoclonal antibody directed against human rhinovirus serotype 2, J Biol Chem 265, 16799-800.

Tormo, J., Stadler, E., Skern, T., Auer, H., Kanzler, O., Betzel, C., Blaas, D., and Fita, I. (1992). Three-dimensional structure of the Fab fragment of a neutralizing antibody to human rhinovirus serotype 2, Protein Sci 1, 1154-61.

Verdaguer, N., Blaas, D., and Fita, I. (2000). Structure of human rhinovirus serotype 2 (HRV2), J Mol Biol 300, 1179-94.


Verdaguer, N., Marlovits, T. C., Bravo, J., Stuart, D. I., Blaas, D., and Fita, I. (1999). Crystallization and preliminary X-ray analysis of human rhinovirus serotype 2 (HRV2), Acta Crystallogr D Biol Crystallogr 55 ( Pt 8), 1459-61.

Vlasak, M., Blomqvist, S., Hovi, T., Hewat, E., and Blaas, D. (2003). Sequence and structure of human rhinoviruses reveal the basis of receptor discrimination, J Virol 77, 6923-30.


Zauner, W., Blaas, D., Kuechler, E., and Wagner, E. (1995). Rhinovirus-mediated endosomal release of transfection complexes, J Virol 69, 1085-92.