Gottfried Köhler Lab

Biomolekulare Optische Spektroskopie/Biomolecular optical spectroscopy
 

The biophysical characterization of interactions between biomolecules in solution as well as on a live cell level represents the main object of our research. Methods include fluorescence and time resolved fluorescence techniques. Dynamic studies are performed in a wide range of time resolution based on a pulsed NdYAG laser for nanosecond transient absorption spectroscopy and a state of the art “stopped flow” system using fluorescence and circular dichroism detection.

Studies on a single molecule level are performed using fluorescence correlation spectroscopy (FCS) and other correlation methods are applied e.g. ligand – receptor interactions in single living cells or virus – receptor interactions. Thermodynamic studies by optical spectroscopy are complemented by biocalorimetry (DSC). Several biological systems are studied in close cooperation with other research groups.