GROUP SCHROEDER | Biochemistry & Biophysics, Genetics, Epigenetics & Gene Regulation
RNA Aptamers and RNA Chaperones
Dr. Bohr-Gasse 9, 1030 Vienna | Room: 5.113
Overview of SELEX procedure. A genomic RNA library derived from DNA is submitted to several rounds of selection (b-e) and amplification (f-g,a) until a pool enriched in desired sequences is obtained. Deep sequencing of enriched pools allows the annotation of genomic aptamers to analysed genomes.
RNA is at the center of all steps of gene expression. Cells can be defined by their transcriptomes, not by their genomes. We are interested in discovering many regulatory elements that are part of the RNA regulon and in identifying their interacting partners and their targets. To achieve this goal we adapted the classical SELEX procedure to be used in combination with genome sequences and deep sequencing. Genomic systematic evolution of ligands by exponential enrichment (SELEX) allows the isolation of protein binding RNAs independently of computational predictions and expression conditions. We...