GROUP WARREN | Biochemistry & Biophysics, Molecular Cell Biology
Biogenesis of the Golgi apparatus
Early in the cell cycle (left panel) the old Golgi (G; red) in T. brucei is located near to one lobe of a bi-lobe structure (green, closed arrowheads). Later in the cell cycle (right panel) the new Golgi is found associated with the other lobe suggesting that the bi-lobe has a role to play in the duplication process. N=nucleus; K=kinetoplast (mitDNA); open arrowheads=basal bodies.
During normal growth and division, cells double in mass and divide into two equally-sized daughters. All cell constituents must be duplicated and then segregated equally during mitosis. For some constituents, such as the chromosomes, the underlying principles and the mechanistic details are relatively clear. For membrane-bound organelles, such as the Golgi, the principles and mechanism have been controversial. The primary aim of our research work is to understand how the cell creates another copy of the Golgi during the cell cycle and partitions them equally between the two daughter cells,...