Group Jantsch Michael

Impact of RNA-editing on coding and non coding substrate RNAs

The Michael Jantsch group has relocated to the Center of Anatomy and Cell Biology at the Medical University of Vienna. The lab can be found on Schwarzspanierstrasse 17, 1090 Vienna.

RNA-editing by adenosine deaminases acting on RNA (ADARs) leads to the post-transcriptional conversion of adenosines to inosines. As inosines are interpreted as guanosines by most cellular processes this type of editing can affect the coding potential of an RNA, its folding, stablility, or localization. ADAR mediated editing is widespread in metazoa and affects thousands of transcripts in the human transcriptome. Together with alternative splicing, RNA-editing therefore leads to massive diversification of the proteome. This is exemplified best by the fact that both RNA-editing, and alternative splicing are most abundant in the mammalian brain. Consistent with its important function in modulating the transcriptome, RNA editing is essential for normal life and development in many organisms. Our research is focused on topics related to this type of RNA editing and aims at understanding the biochemical, cellular, and organismic consequences of A to I conversion.

Editing in protein coding mRNAs

A handful of highly conserved protein coding targets for A to I editing are known today. To understand the impact of editing on these RNAs and their encoded proteins we are generating transgenic mice that are impaired in specific editing events. Our studies show that lack of editing of the mRNAs encoding the actin crosslinking protein filamin A leads to behavioral defects disturbed neuronal outgrowth underscoring the importance of editing for proper neuronal function.

Publications since 2006

Licht, Konstantin; Jantsch, Michael F (2016). Rapid and dynamic transcriptome regulation by RNA editing and RNA modifications. J CELL BIOL;213(1):15-22. PMID: 27044895

Cusanelli, Emilio; Chartrand, Pascal (2015). Telomeric repeat-containing RNA TERRA: a noncoding RNA connecting telomere biology to genome integrity. Frontiers in genetics;6:143. PMID: 25926849

Barraud, Pierre; Banerjee, Silpi; Mohamed, Weaam I; Jantsch, Michael F; Allain, Frédéric H-T (2014). A bimodular nuclear localization signal assembled via an extended double-stranded RNA-binding domain acts as an RNA-sensing signal for transportin 1. P NATL ACAD SCI USA;111(18):E1852-61. PMID: 24753571

Muggenhumer, Dominik; Vesely, Cornelia; Nimpf, Simon; Tian, Nan; Yongfeng, Jin; Jantsch, Michael F (2014). Drosha protein levels are translationally regulated during Xenopus oocyte maturation. MOL BIOL CELL;25(13):2094-104. PMID: 24829383

Vesely, Cornelia; Tauber, Stefanie; Sedlazeck, Fritz J; Tajaddod, Mansoureh; Haeseler, Arndt von; Jantsch, Michael F (2014). ADAR2 induces reproducible changes in sequence and abundance of mature microRNAs in the mouse brain. NUCLEIC ACIDS RES;42(19):12155-68. PMID: 25260591

Mannion, Niamh M; Greenwood, Sam M; Young, Robert; Cox, Sarah; Brindle, James; Read, David; Nellåker, Christoffer; Vesely, Cornelia; Ponting, Chris P; McLaughlin, Paul J; Jantsch, Michael F; Dorin, Julia; Adams, Ian R; Scadden, A D J; Ohman, Marie; Keegan, Liam P; O'Connell, Mary A (2014). The RNA-Editing Enzyme ADAR1 Controls Innate Immune Responses to RNA. Cell Rep;9(4):1482-94. PMID: 25456137

Tariq, Aamira; Garncarz, Wojciech; Handl, Cornelia; Balik, Ales; Pusch, Oliver; Jantsch, Michael F (2013). RNA-interacting proteins act as site-specific repressors of ADAR2-mediated RNA editing and fluctuate upon neuronal stimulation. NUCLEIC ACIDS RES;41(4):2581-93. PMID: 23275536

Garncarz, Wojciech; Tariq, Aamira; Handl, Cornelia; Pusch, Oliver; Jantsch, Michael F (2013). A high throughput screen to identify enhancers of ADAR-mediated RNA-editing. RNA BIOL;10(2):190-202. PMID: 23353575

Jantsch, Michael F (2013). Editing the flow of information. RNA BIOL;10(10):1609-1610.

Stuli?, Maja; Jantsch, Michael F (2013). Spatio-temporal profiling of Filamin A RNA-editing reveals ADAR preferences and high editing levels outside neuronal tissues. RNA BIOL;10(10):1611-7. PMID: 24025532

Vesely, Cornelia; Tauber, Stefanie; Sedlazeck, Fritz J; von Haeseler, Arndt; Jantsch, Michael F (2012). Adenosine deaminases that act on RNA induce reproducible changes in abundance and sequence of embryonic miRNAs. GENOME RES;22(8):1468-76. PMID: 22310477

Tariq, Aamira; Jantsch, Michael F (2012). Transcript diversification in the nervous system: a to I RNA editing in CNS function and disease development. Frontiers in neuroscience;6:99. PMID: 22787438

Tian, Nan; Yang, Yun; Sachsenmaier, Nora; Muggenhumer, Dominik; Bi, Jingpei; Waldsich, Christina; Jantsch, Michael F; Jin, Yongfeng (2011). A structural determinant required for RNA editing. NUCLEIC ACIDS RES(39):5669-81. PMID: 21427087

Tang, Lois; Machacek, Thomas; Mamnun, Yasmine M; Penkner, Alexandra; Gloggnitzer, Jiradet; Wegrostek, Christina; Konrat, Robert; Jantsch, Michael F; Loidl, Josef; Jantsch, Verena (2010). Mutations in Caenorhabditis elegans him-19 show meiotic defects that worsen with age. MOL BIOL CELL;21:885-896. PMID: 20071466

Pullirsch, Dieter; Jantsch, Michael F (2010). Proteome diversification by adenosine to inosine RNA editing. RNA BIOL. PMID: 20200492

Jantsch, Michael F (2010). Reaching complexity through RNA editing. RNA BIOL. PMID: 20534976

Fritz, Jutta; Strehblow, Alexander; Taschner, Andreas; Schopoff, Sandy; Pasierbek, Pawel; Jantsch, Michael F (2009). RNA-regulated interaction of transportin-1 and exportin-5 with the double-stranded RNA-binding domain regulates nucleocytoplasmic shuttling of ADAR1. MOL CELL BIOL. PMID: 19124606

Pouch-Pélissier, Marie-Noëlle; Pélissier, Thierry; Elmayan, Taline; Vaucheret, Hervé; Boko, Drasko; Jantsch, Michael F; Deragon, Jean-Marc (2008). SINE RNA induces severe developmental defects in Arabidopsis thaliana and interacts with HYL1 (DRB1), a key member of the DCL1 complex. PLOS GENET. PMID: 18551175

Riedmann, Eva M; Schopoff, Sandy; Hartner, Jochen C; Jantsch, Michael F (2008). Specificity of ADAR-mediated RNA editing in newly identified targets. RNA. PMID: 18430892

Schoft, Vera K; Schopoff, Sandy; Jantsch, Michael F (2007). Regulation of glutamate receptor B pre-mRNA splicing by RNA editing. NUCLEIC ACIDS RES. PMID: 17517775