Group Köhler Gottfried

Biomolecular optical spectroscopy

Biophysical characterisation of biomolecules and of their interactions in solution as well as on a live cell level represents the main object of our research. Methods include fluorescence and time resolved techniques performed over a wide range of time resolution. Studies by optical spectroscopy are complemented by biocalorimetry (DSC).

Quantitative studies on molecular dynamics on a single molecule level are performed using advanced fluorescence correlation techniques. Among others, these methods are applied on studies of ligand-receptor interactions relevant for hormone regulation and of the mechanisms of endocytosis and transport in single living cells. These measurements provide the basis for mathematical modelling of complex dynamic behaviour in bio-systems, implemented in close cooperation with other research groups.

Translational research related to clinical diagnostics is another area of focus. Our aim is to establish and to optimize novel and highly sensitive detection tools for inflammatory biomarkers such as interleukins in body fluids (serum, saliva and tears) using fluorescence correlation techniques. It will help to develop cost effective, highly sensitive and portable diagnostic/screening devices that can be incorporated into clinics. Other project deals with the detection of malignant cells in body fluids such as urine by fluorescence imaging. This technology will also allow to detect circulating malignant cells (CTC) in plasma in case of cancer metastasis.

Publications since 2006

Keller, Amy C; Badani, Hussain; McClatchey, P Mason; Baird, Nicholas L; Bowlin, Jacqueline L; Bouchard, Ron; Perng, Guey-Chuen; Reusch, Jane E B; Kaufer, Benedikt B; Gilden, Don; Shahzad, Aamir; Kennedy, Peter G E; Cohrs, Randall J (2016). Varicella zoster virus infection of human fetal lung cells alters mitochondrial morphology. Journal of neurovirology.. PMID: 27245593

Rieder, Franz Jj; Kastner, Marie-Theres; Hartl, Markus; Puchinger, Martin G; Schneider, Martina; Majdic, Otto; Britt, William J; Djinovic-Carugo, Kristina; Steininger, Christoph (2016). Human cytomegalovirus phosphoproteins are hypophosphorylated and intrinsically disordered. J GEN VIROL. PMID: 27959783

Shushan Harutyunyan,Arthur Sedivy,Gottfried Köhler,Heinrich Kowalski,Dieter Blaas (2015). Application of FCS in Studies of Rhinovirus Receptor Binding and Uncoating. Methods Mol Med;1221:83-100. PMID: 25261309

Cieślik-Boczula, Katarzyna; Swiątek, Piotr; Jaszczyszyn, Agata; Zawilska, Patrycja; Gąsiorowski, Kazimierz; Malinka, Wiesław; Köhler, Gottfried (2014). Phase separation in phosphatidylcholine membrane caused by the presence of a pyrimidine analogue of fluphenazine with high anti-multidrug-resistance activity. J PHYS CHEM B;118(13):3605-15. PMID: 24601791

Alaghaz, Abdel-Nasser M. A.; Ammar, Reda A. A.; Koehler, Gottfried; Wolschann, Karl Peter; El-Gogary, Tarek M. (2014). Synthesis, spectral and quantum chemical studies on NO-chelating sulfamonomethoxine-cyclophosph(V)azane and its Er(III) complex. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy.;128:724-729. PMID: 24699291

Chen Shen, Martin Knapp, Martin Gerald Puchinger, Aamir Shahzad, Erwin Gaubitzer, A Dong Shen, Gottfried koehler (2014). Using fluorescence correlation spectroscopy (FCS) for IFN-g detection: A preliminary study Journal of immunological methods.;407:35-39. PMID: 24685834

Harutyunyan S, Kumar M, Sedivy A, Subirats X, Kowalski H, Koehler G, Blaas D (2013). Viral Uncoating Is Directional: Exit of the Genomic RNA in a Common Cold Virus Starts with the Poly-(A) Tail at the 3′-End. PLOS PATHOG;9(4):e1003270. PMID: 23592991

Zarzer, Clemens A; Puchinger, Martin G; Köhler, Gottfried; Kügler, Philipp (2013). Differentiation between genomic and non-genomic feedback controls yields an HPA axis model featuring Hypercortisolism as an irreversible bistable switch. Theoretical biology & medical modelling;10:65. PMID: 24209391

Rangger C, Helbok A, Sosabowski J, Kremser C, Koehler G, Prassl R, Andreae F, Virgolini IJ, von Guggenberg E, Decristoforo C (2013). Tumor targeting and imaging with dual-peptide conjugated multifunctional liposomal nanoparticles International journal of nanomedicine.;8(1):4659 - 4671. PMID: 24353415

Tarek M. El-Gogary, Abdullah G. Al-Sehemi, Karl Peter Wolschann, and Gottfried Koehler (2013). Structure and Stability of Chemically Modified DNA Bases: Quantum Chemical Calculations on 16 Isomers of Diphosphocytosine. ISRN Physical Chemistry. PMID: 24138259

Michael Edetsberger, Martin Knapp, Erwin Gaubitzer, Christoph Miksch. Kathuna Elizbarowna Gvichiya, Gottfried Koehler (2012). Effective staining of tumor cells by coumarin-6 depends on the stoichiometry of cyclodextrin complex formation J Incl Phenom Macrocycl Chem. ;70(3):327-331.

Agócs, Gergely; Szabó, Bence T; Köhler, Gottfried; Osváth, Szabolcs (2012). Comparing the Folding and Misfolding Energy Landscapes of Phosphoglycerate Kinase. BIOPHYS J;102(12):2828-34. PMID: 22735533

Puchinger,Martin Gerald; Zarzer, Clemens Alexander; Kügler,Philip; Gaubitzer, Erwin; Köhler, Gottfried (2012). In vitro detection of adenocorticotropic hormone levels by fluorescence correlation spectroscopy immunoassay for mathematical modeling of glucocorticoid-mediated feddback mechanisms. EURASIP journal on bioinformatics & systems biology;17. PMID: 23102048

Aamir Shahzad, Gottfried Köhler (2011). Fluorescence Correlation Spectroscopy (FCS): A Promising Tool for Biological Research APPL SPECTROSC REV;46(2):166-173.

Aamir Shahzad, Craig S McLachlan, Judith Gault, Randall J Cohrs, Xiangdong Wang and Gottfried Köhler (2011). Global translational medicine initiatives and programs Translat. Biomed. ;2(3:2).

Hiroi, Noriko; Lu, James; Iba, Keisuke; Tabira, Akito; Yamashita, Shuji; Okada, Yasunori; Flamm, Christoph; Oka, Kotaro; Köhler, Gottfried; Funahashi, Akira (2011). Physiological environment induces quick response - slow exhaustion reactions. Front Physiol;2:50. PMID: 21960972

Aamir Shahzad, Randall J Cohrs, Roland Andersson, Xiangdong Wang and Gottfried Köhler (2011). Recommendations for Comprehensive Translational Medicine Education and Training Translat. Biomed. ;2(2:1).

Shahzad, Aamir; Knapp, Martin; Lang, Irene; Köhler, Gottfried (2011). The use of fluorescence correlation spectroscopy (FCS) as an alternative biomarker detection technique: a preliminary study. J CELL MOL MED;15(12):2706-11. PMID: 21306559

Edetsberger Michael, Gaubitzer Erwin, Miksch Christoph, Knapp Martin, Balluch Bruno, Ggvichiya Elizbarowna, Atassi Ibrahim, Smetana Walter, Köhler Gottfried (2011). Detection of Malign Cells in Bio-Fluids for Diagnostic Purposes. Proceedings E!4570 IPCTECH:19-26.

Edetsberger Michael, Atassi Ibrahim, Smetana Walter, Köhler Gottfried (2011). A Biodevice for Real Time Cell Observation Fabricated in LTCC Technology. Proceedings E!4570 IPCTECH:27-32.