BD FACS Aria IIIu

Location: MFPL Main Building (Dr.-Bohr-Gasse 9) - Level 2, Room 2.126

The BD FACS ARIA IIIu is a 5 laser cell sorter with 16 fluorescence detectors, which allows sorts of up to 4 cell populations simultaneously as well as single cell sorting into multi-well plates. Temperature control for sample input and sort output (4°C to room temperature) is possible.

Laser 1

375 nm Near UV Laser

Detection

450/40 nm –DAPI, Hoechst
670LP nm – Hoechst Red

Laser 2

405 nm Violet Laser, 50 mW

Detection

450/40 nm – BV421, Alexa Fluor 405, DAPI, Hoechst, Pacific Blue, BFP
525/50 nm – BV510, Alexa Fluor 430, Horizon V500, Pacific Orange
610/20 nm – BV605
660/20 nm – BV650
710/50 nm - BV711
780/60 nm - BV786

Laser 3

488 nm Blue Laser, 50 mW

Detection

488/10 nm - SSC
530/30 nm - FITC, GFP, YFP, CFP
, Venus, Alexa Fluor 488
695/40 nm - PerCP-Cy5.5

Laser 4

561 nm Yellow Green Laser, 50mW

Detection

582/15 nm - PE, DsRed
610/20 nm - PE-Texas Red, PI, mCherry
670/14 nm – PE-Cy5
710/50 nm - PE-Alexa Fluor 700, PE-Cy5.5
780/60 nm – PE-Cy7

Laser 5

633 nm Red laser, 40 mW

Detection

675/20 nm - APC, Alexa Fluor 647
730/45 nm - Alexa Fluor 700
780/60 nm - APC-Cy7

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