BD LSR Fortessa

Location: MFPL Main Building (Dr. Bohr-Gasse 9) - Level 2, Room 2.126

The FACS LSR Fortessa is a 4 laser flow cytometer with 16 fluorescence detectors, which is additionally equipped with a HTS (High Throughput Sampler) for 96 well and 384-well plates.

Laser 1

405 nm Violet Laser, 50 mW

Detection

450/50 nm – BV421, Alexa Fluor 405, DAPI, Hoechst, Pacific Blue, BFP
525/50 nm – BV510, Alexa Fluor 430, Horizon V500, Pacific Orange
610/20 nm – BV605
670/30 nm – BV650
710/50 nm - BV711
780/60 nm - BV786

Laser 2

488 nm Blue Laser, 50 mW

Detection

488/10 nm - SSC
530/30 nm - FITC, GFP, YFP, CFP
, Venus, Alexa Fluor 488
710/50 nm - PerCP-Cy5.5, PerCP

Laser 3

561 nm Yellow Green Laser, 50mW

Detection

586/15 nm - PE, DsRed
610/20 nm - PE-Texas Red, PI, mCherry
670/30 nm – PE-Cy5, 7-AAD
710/50 nm - PE-Alexa Fluor 700, PE-Cy5.5
780/60 nm – PE-Cy7

Laser 4

640 nm Red laser, 40 mW

Detection

670/14 nm - APC, Alexa Fluor 647
730/45 nm - Alexa Fluor 700
780/60 nm - APC-Cy7
 

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