BioOptics - Light Microscopy
"Learn to live with Uncertainty"
The BioOptics facility is dedicated to providing state-of-the art light microscopy equipment to MFPL researchers.The facility has been centralized in rooms of the VBC5-building. Currently, the following microscopes are available: two laser scanning confocals, a STED super-resolution microscope, two spinning disk units, a live-imaging station equipped for TIRF with full environmental control and microfluidics, two epifluorescence deconvolution microscopes, two high-content imaging stations and a microdissection-laser ablation instrument. The facility management provides professional training and assists in experimental planning, technical setup, troubleshooting and image analysis.
The new DELTAVISION "ULTRA" deconvolution microscopy system installed and ready for use
(November 20th 2018). The new deconvolution widefield fluorescence microscopy system release from GE -"Deltavision Ultra"- has been installed after thorough testing and is now ready for training and usage. It is a refurbished, technically upgraded version of our existing Deltavision microscope. The major new technical features include a new acquisition software (Acquire Ultra), a hardware autofocus, a fast and sensitive sCMOS camera, a 60 x silicone objective, a new stage to hold slides, dishes AND multi-well plates. Finally we have invested in a fully environmentrally controlled setup for live imaging approaches.
New Zeiss "Celldiscoverer 7" high content microscopy system installed and open for use
(July 25th 2018). Our new high-content system "Celldiscoverer" has been installed, successfully tested and is now available for usage. It represents an “all-in-a-box” configured system, dedicated for long-term and high-content imaging. The major new technical features include automatic detection of sample containers and measurement of specimen-specific physical parameters (RI, bottom composition and thickness). The latter are used to adjust auto-corrected objectives to allow optical optimization for the respective setup. An intricate focus detection hierarchy allows the user to programme easy to very complex imaging routines.
Location and Rooms
VBC5 - Structural and Computational Biology Dept., Level E1
- Office: room 1.618 (tel. ext. 61672)
- Microscopes 1: room 1.223 (tel. ext. 61678)
- Microscopes 2: room 1.320 (tel. ext. 61677)
- Microscopes 3: room 1.318 (tel. ext. 61679)
- Microscopes 4: room 1.723 (CIBIV; tel.ext. 61675)
- Tissue Culture: room 1.219(tel.ext. 52247)
- Image Processing workstations: MFPL main building, 6th floor, room 6.508.
E-mail: lightmicroscopy [AT] mfpl.ac [DOT] at
Josef Gotzmann: +43-664-8001635200
Thomas Peterbauer: +43-664-60277-52878
Available Equipment (room #)
- Inverse confocal Zeiss LSM 710 with Airyscan detector (1.223)
- Inverse confocal Zeiss LSM 700 (1.223)
- Inverse live imaging platform Zeiss Celldiscoverer 7 (1.320)
- Upright Abberior Instruments STEDYCON for STED (1.723)
- Inverse Visitron Spinning Disk unit with FRAP (1.223)
- Inverse Live Spinning Disk/Nanodissection unit (1.318)
- Inverse Deltavision epifluorescence microscope (1.320)
- Inverse Deltavision Ultra epifluorescence microscope (1.320)
- Inverse live imaging unit Olympus cellSens /TIRF (1.223)
- Upright microdissection unit Leica LMD6500 (1.723)
- Inverse High Content Microscope unit (HCM) (1.723)
- Two image processing workstations (room 6.508, MFPL main building) providing stand-alone microscope software (Olympus cellSens, ZEN 2012, Visiview) and professional software packages for deconvolution and 3/4D image processing (SVI Huygens, softWoRx suite)
- HIVE acquisition server for remote saving of images (access instructions)
- Zeiss Stereomicroscope Stemi2000 (transmitted/incident light; room 1.320)
- Leica Stereomicroscope S6E (transmitted/incident light; room 1.318)
- BSL2 Tissue Culture Hood (room 1.219)
- BSL1 Tissue Culture (room 1.219, hood, microscope, incubator, freezer & fridge)
Potential trainees must
- provide an organized experimental strategy to discuss with the facility staff
- already have own samples for a specialized training session
- Fill in the "Light Microscopy Training Application" form here and send it via lightmicroscopy [AT] mfpl.ac [DOT] at to organize a meeting with the facility staff. We then discuss most forward strategies and find the proper microscope(s) to be trained on.*
- Attend the "Introductory Lecture Biooptics" including laser safety instructions.**
- Organize a training unit with the facility staff – training units will be split into "how to do" and "optimize my sample" sessions (on separate days).
- Fill in and sign the “Training Confirmation” form on usage of microscopes/user fee regulations (trainee and group leader) and bring it to your training session.
- Before you attend the sessions, please download our "General Administrative Rules" and read them thoroughly!
*Optional: facility personnel evaluates potential applicability with user specific samples, if selection of the proper microscope system remains unclear.
**1.) and 2.) may be switched.
Lectures always take place in the seminar rooms in VBC5, level E1 (Structural Biology Dept.). Lecture Dates are announced regularly. To register, login to the MFPL Intranet ⇒ "Booking System" ⇒ ”Lecture and Training Registration".
User Fee Strategy
MFPL users can access current prices via the intranet ("Booking System" ⇒ "Facility Infos"). External users in any case need to contact the head of facility, Josef Gotzmann, to get informed on conditions to use MFPL's Biooptics instrumentation.
In House Microscopes
Five microscope systems (one in each floor + teaching microscope) in the MFPL main building have been upgraded to state-of-the-art technology. They are fully motorized, host high-end CCD cameras and are fully equipped for fluorescence/brightfield imaging. Irmgard Fischer is the responsible person for these microscopes: she helps in training and troubleshooting and maintenance/service.
Irmgard Fischer; Facility Technician; Rooms 4.621/5.506; Tel. ext. 52866;
mobile:0660/7681381; Irmgard.fischer [AT] univie.ac [DOT] at
- 2nd floor (room 2305) - Axiovert 200M
- 3rd floor (room 3517) - Axio Observer Z1
- 4th floor (room 4407) - Axio Imager Z2
- 5th floor (room 5423) - Axio Imager M2
- Teaching (room 5519) - Axio Observer Z1
Joachim Garbrecht, Harald Hornegger, Sebastien Herbert, Tanja Kaufmann, Josef Gotzmann, Kareem Elsayad and Dea Slade: "Simultaneous dual-channel imaging to quantify interdependent protein recruitment to laser- induced DNA damage sites." Nucleus (2018), 9(1), pp.474-491. https://doi.org/10.1080/19491034.2018.1516485
Gabriele Zaffagnini, Adriana Savova, Alberto Danieli, Julia Romanov, Shirley Tremel, Michael Ebner, Thomas Peterbauer, Martin Sztacho, Riccardo Trapannone, Abul K Tarafder, Carsten Sachse and Sascha Martens : "Phasing out the bad-How SQSTM1/p62 sequesters ubiquitinated proteins for degradation by autophagy." Autophagy (2018), 14(7), pp.1280-1282. https://doi.org/10.1080/15548627.2018.1462079
Gabriele Zaffagnini, Adriana Savova, Alberto Danieli, Julia Romanov, Shirley Tremel, Michael Ebner, Thomas Peterbauer, Martin Sztacho, Riccardo Trapannone, Abul K Tarafder, Carsten Sachse and Sascha Martens : "p62 filaments capture and present ubiquinated cargos for autophagy ." EMBO Journal (2018), 37(5), e98308. http://onlinelibrary.wiley.com/doi/10.15252/embj.201798308/abstract
Tanja Kaufmann, Irina Grishkovskaya, Anton A. Polyansky, Sebastian Kostrhon, Eva Kukolj, Karin M. Olek, Sebastien Herbert, Etienne Beltzung, Karl Mechtler, Thomas Peterbauer, Josef Gotzmann, Lijuan Zhang, Markus Hartl, Bojan Zagrovic, Kareem Elsayad, Kristina Djinovic-Carugo and Dea Slade: "A novel non-canonical PIP-box mediates PARG interaction with PCNA." Nucleic Acids Research (2017), 45(16), pp.9741–9759, https://doi.org/10.1093/nar/gkx604