Available Equipment

LSM 710 Airyscan

Inverse point scanning confocal microscope with 3 PMT channels and Airyscan detector (compound detector of 32 sensitive GaAsP units) for super-resolution imaging. Spectral imaging (lambda scanning, linear unmixing) via sequential acquisition, transmitted light PMT and Piezo-driven XY stage with Z-Piezo insert (optional). Widefield illumination: halogen and X-Cite 120 XL short-arc lamps. Experiment Designer module for automated acquisition and complex workflows. Environmental control (stage-based) possible.

Objectives

Lasers

  • 405 nm laser diode (30 mW)
  • 458, 477, 488 and 514 nm Argon laser (25 mW)
  • 561 nm DPSS laser (20 mW)
  • 633 nm HeNe laser (5 mW)

Downloads

LSM 700

Inverse point scanning confocal microscope with 2 PMT channels and a variable secondary dichroic (permitting spectral imaging). Transmitted light detector, motorized stage. Widefield illumination: halogen lamp, CoolLED pE-2.

Objectives

  • EC Plan-Neofluar 10x/0.3
  • LD LCI Plan-Apochromat 25x/0.8 Imm Corr (oil, glycerol, water)
  • Plan-Apochromat 40x/1.3 Oil DIC
  • Plan-Apochromat 63x/1.4 Oil DIC

Lasers

  • 405, 488, 555 and 639 nm laser diodes
  • 5 mW (405, 639 nm) or 10 mW (488, 555 nm)

Downloads

STEDYCON

Upright point scanning microscope with 2 avalanche photodiodes for dual-color 2D STED (orange/dark red) or conventional triple-color confocal imaging (green, orange and dark red). Piezo-driven z-focus for 3D acquisitions, manual XY stage for standard slides. Widefield illumination: CoolLED pE-2, halogen lamp.

Objectives

Lasers

  • 488 nm (for confocal imaging)
  • 561 nm (for confocal and STED imaging)
  • 640 nm (for confocal and STED imaging)
  • 775 nm (STED depletion laser)

Recommended STED dyes and their theoretical resolution limit

  • Abberior
    STAR 580 (60 nm), STAR 600 (72 nm), STAR 635 (42 nm), STAR 635P (41 nm), STAR RED (41 nm)
  • Alexa Fluor
    594 (60 nm), 647 (30 nm)
  • ATTO
    590 (60 nm), 633 (42 nm), 647N (42 nm)

Downloads

Visitron Spinning Disk 1

Inverse spinning disk microscope of fast pseudo-confocal imaging. Equipped with a Yokogawa CSU-X1 spinning disk, a standard CCD camera (CoolSNAP HQ2) and a sensitive EM-CCD camera (Hamamatsu ImageEM X2). Motorized stage, hardware autofocus, a 405 nm point scanner option for FRAP/photoactivation. Widefield illumination: halogen lamp, HXP short-arc lamp. Optional: ultra-fast CherryTemp heater/cooler for precise temperature control and temperature-shift experiments in the range of 5-45°C.

Objectives

  • LD Achroplan 20x/0.4 Corr
  • Plan-Apochromat 63x/1.4 Oil DIC

Lasers

  • 405 nm laser diode (120 mW, 50/50 power split for imaging or FRAP)
  • 488 nm diode (100 mW)
  • 561 nm DPSS laser (100 mW)

Downloads

Visitron LIVE Nanodissection Unit

Inverse spinning disk unit with iLAS Pulse FRAP module for photomanipulation (FRAP, photoactivation, photoconversion). All lasers can be used for photomanipulation. Pulsed 355 nm laser for nanodissection. Multi-positioning, box-based environmental control and hardware autofocus. Equipped with a sensitive EM-CCD camera for long-term live imaging (Hamamatsu ImageEM X2) and an sCMOS camera for fast imaging at high resolution (Hamamatsu ORCA-Flash4.0). Widefield imaging: white LED, solid-state Spectra-X light engine, OptoSplit (for fast, simultaneous dual-channel acquisition), sCMOS camera.

Objectives

  • EC Plan-Neofluar 20x/0.5
  • Plan-Apochromat 63x/1.4 Oil DIC
  • Plan-Neofluar 63x/1.25 Oil
  • EC Plan-Neofluar 100x/1.3 Oil Iris

Lasers

  • 405 nm laser diode (120 mW)
  • 488 nm laser diode (100 mW)
  • 561 nm DPSS laser (100 mW, AOM-controlled)
  • 640 nm laser diode (150 mW)
  • 355 nm pulsed laser (average power 16 mW, 21 kHz, 0.8 µJ/pulse) for nanodissection

Downloads

Celldiscoverer

Platform for high-throuput live imaging. Accomodates multiwell plates, dishes and chamber slides with glass or plastic bottom. Equipped with hardware autofocus, autocorr objectives (50x with water immersion), magnification changer (up to 2x), 6-Megapixel CCD and 4-Megapixel sCMOS camera. Illumination: 7-color LED for fluorescence (all dyes from deep blue to far red), IR-LED for transmitted light and phase gradient contrast. Temperature/CO2 control, dispensing unit for manual reagent addition, and UV disinfection. Python interface for real-time image analysis and custom workflows.

Objectives

  • Plan-Apochromat 5x/0.35 (glass or plastic bottom)
  • Plan-Apochromat 20x/0.7 autocorr (glass or plastic bottom)
  • Plan-Apochromat 50x/1.2 W autocorr (glass or plastic bottom)

Downloads

personal DeltaVision

Inverse widefield epifluorescence microscope equipped with CoolSNAP HQ2 camera, motorized stage and integrated deconvolution algorithms. Illumination: 7-color solid state illumination source, white LED.

Objectives

  • UPlan SApo 20x/0.85 Oil
  • UApo N 340 40x/1.35 Oil
  • Plan Apo N 60x/1.42 Oil
  • UPlan SApo 100x/1.40 Oil

Downloads

Olympus cellSens

Inverse widefield microscope with full environmental control for live imaging, DIC optics, LED light source with 6 excitation bands, TIRF and single-point FRAP option. Hamamatsu ORCA-Flash4.0 sCMOS camera, ImagEM X2 EM-CCD or Olympus SC50 color CMOS camera. Motorized stage, z-drift compensation (hardware autofocus) and an ONIX microfluidic system (CellASIC).

Objectives

  • UPlanSApo 4x/0.16
  • UPlanSApo 10x/0.4
  • UPlanSApo 20x/0.75
  • UApo/340 40x/1.35 Oil Iris
  • PlanApo N 60x/1.42 Oil
  • UApo N 100x/1.49 Oil TIRF Corr

Lasers

  • 405 nm laser diode (100 mW, TIRF or single-point FRAP)
  • 488 nm laser diode (100 mW, TIRF)
  • 561 nm DPSS laser (100 mW, TIRF)

Downloads

Olympus IX81 HCM

Inverse widefield microscope with phase contrast optics and long-distance objectives for cell culture dishes (up to 40x). Olympus MT20 Xenon arc lamp and filter sets to image all common dyes, motorized XY stage. Scriptable for arbitrary workflows, including acquisition with feedback from real-time image analysis. Stage-based environmental control on request.

Objectives

  • UPlanFL N 4x/013 PhL (working distance 17 mm)
  • UPlanFL N 10x/0.3 Ph (WD 10 mm)
  • UPlanFL N 20x/0.5 Ph (WD 2.1 mm)
  • LUCPlanFL N 40x/0.6 Ph Corr (WD 3.0-4.2 mm)
  • UPlanFL N 40x/0.75 Ph (WD 0.51 mm)
  • UPlanFL N 60x/1.25 Oil Ph Iris (WD 0.1 mm)

Downloads

LMD6500

Upright, motorized microdissection system with a 355 nm pulsed DPSS laser. Equipped with a Leica DFC300 FX color camera, a halogen lamp and a metal halide lamp for bright field and epifluorescence imaging.

Objectives

  • UVI 5x/0.12
  • HC PL Fluotar 10x/0.3
  • HCX PL Fluotar 20x/0.4 Corr
  • HCX PL Fluotar L 40x/0.6 Corr XT
  • HCX PL Fluotar L 63x/0.7 Corr XT
  • HCX PL APO 63x/1.2 W Corr
  • HCX PL APO 100x/1.4-0.7 Oil CS

Filter sets

  • Triple band RGB filter (DAPI/FITC/Texas Red)
  • Individual excitation band pass filters for each color

Downloads

Two workstations providing offline versions of proprietary microscope software as well as professional and open source image analysis packages.

Workstation "Company Licences"

  • Zeiss ZEN 2012 SP1 (Black/Blue)
  • Olympus cellSens Dimension Desktop 1.17
  • Applied Precision softWoRx suite 2.0
  • Visitron VisiView 2.0.8
  • ImageJ/Fiji
  • Adope Photoshop, Illustrator CS4, Acrobat Pro 9, Office 2007

Workstation "Deconvolution"

  • Huygens Professional (SVI)
  • ImageJ/Fiji

Downloads

HIVE acquisition server with fast network connections for remote saving of images and image processing.

Connected Microscopes

Hardware

  • Intel Xeon E5-1650 v3 CPU (3.50 GHz, 128 GB RAM)
  • NVIDIA GeForce GTX970 GPU (8 GB RAM)
  • Adaptec 8885 SCSI RAID Controller (10 TB)
  • 2x Intel Ethernet Converged Network Adapter X710 (10 Gb/s)
  • 2x Intel Ethernet Controller X540-AT2 (10 Gb/s)
  • 2x Intel Ethernet Controller I210 (1 Gb/s)
  • Windows Server 2012 R2

Downloads

To maintain samples during image acquisition sessions, we offer basic tissue culture facilities (BS1 and BS2 laminar flow hoods, fridge and freezer space, incubators). For imaging, various filter sets and accessories are available on request. Get the full list here.