Biochemistry & Biophysics | Genetics, Epigenetics & Gene Regulation
Riboregulation of transcription - how RNA controls its own synthesis
renee.schroeder [AT] univie.ac [DOT] at
Dr. Bohr-Gasse 9, 1030 Vienna | Room: 5.113
Life is a selforganized process, that needs to control growth and establish homeostasis in response to very diverse environmental cues. If RNA is to play the role of the molecule that enabled life to emerge,...more
Life is a selforganized process, that needs to control growth and establish homeostasis in response to very diverse environmental cues. If RNA is to play the role of the molecule that enabled life to emerge, it should be able to control its own synthesis.
Our laboratory is interested in identifying RNAs that interact with RNA polymerases to control transcription. We are currently studying RNA polymerase-binding RNA aptamers (RAPs) that are able to cross talk to RNA polymerases in cis and modulate the transcription of nascent RNAs hosting these RAPs. By performing genomic SELEX using RNA libraries derived from E. coli, yeast and human genomic DNA and highly purified RNA polymerases as bait, we isolated thousands of RAPs. RAP-mediated control of transcription elongation and termination is a novel level of regulation that is conserved from bacteria to humans.
RNAs can control gene expression at all steps. We are specially interested in antisense RNA mediated regulation of the transcriptome via double-stranded RNA formation and transcription interference.
The human transcriptome is modulated by unconventional splicing events like nested and recursive splicing. We are currently addressing the role of intronic splicing on the efficiency of formation of mature RNA.
We recently revealed a novel function for small RNAs in the regulation of transcription in E. coli. A large number of E. coli genes have long 5’ UTRs whose transcription is turned off prematurely by the RNA helicase Rho under exponential growth. Under stress conditions small RNAs are synthesized that bind to these 5’ UTRs competing with rho for interaction with the RNA. By doing this they induce antitermination of transcription leading to full length gene expression. Thus sRNA not only regulate gene expression post-transcriptionally but already co-transcriptionally.
Video: A science-communication film by Nadezda Sedlyarova, Andrés Magán Garcia and Natascha Hartl (University of Vienna), highlighting the importance of basic research in bacteria. A WTZ Ost production (2017) in cooperation with Okto Community TV supported by Austria Wirtschaftsservice and the Federal Ministry of Science, Research and Economy.
Sedlyarova, Nadezda; Shamovsky, Ilya; Bharati, Binod K; Epshtein, Vitaly; Chen, Jiandong; Gottesman, Susan; Schroeder, Renée; Nudler, Evgeny (2016). sRNA-Mediated Control of Transcription Termination in E. coli. CELL;167(1):111-121. PMID: 27662085
Popitsch, Niko; Bilusic, Ivana; Rescheneder, Philipp; Schroeder, Renée; Lybecker, Meghan (2017). Temperature-dependent sRNA transcriptome of the Lyme disease spirochete. BMC GENOMICS;18(1):28. PMID: 28056764
Lybecker, Meghan; Zimmermann, Bob; Bilusic, Ivana; Tukhtubaeva, Nadezda; Schroeder, Renée (2014). The double-stranded transcriptome of Escherichia coli. P NATL ACAD SCI USA;111(8):3134-9. PMID: 24453212
Doctoral Program "RNA Biology"
The Group Schroeder participates in the special Doctoral Program "RNA Biology" reviewed and funded by the Austrian Research Fund FWF.
SFB "RNA-Reg - RNA regulation of the transcriptome"
Renée Schroeder is speaker of the Special Research Area (SFB) "RNA-Reg - RNA regulation of the transcriptome" funded by the Austrian Science Fund FWF. SFB's are peer-reviewed, highly interactive research networks, established to foster long-term, interdisciplinary co-operation of local research groups working on the frontiers of their thematic areas.
Eduard Buchner Prize 2011
German Society for Biochemistry and Molecular Biology (GBM) > view news message
awarded by the Austrian Science Fund FWF on behalf of the Ministry for Science and Research.
Science Book of the Year 2012 and 2017
Read more about Renée Schroeder's prize-winning book here.
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Member of the Austrian Council for Research and Technology
for the function period 2010-2015