BioSorter (Large Particle Flow Cytometer) Union Biometrica

Location: MFPL Main Building (Dr. Bohr-Gasse 9) - Level 2, Room 2.528

The BioSorter® is designed for flow-cytometric analysis and sorting of objects ranging in size from 1 – 1500 µm.

Unlike traditional cytometers, it allows the investigation of viable multicellular organisms, cell clusters, bead-based libraries and other samples that are too large and fragile for standard flow-cytometry.

Parameters that can be analyzed are the relative axial length (TOF, Time of flight), which allows to determine the size of cells/objects, the optical density (EXT, Extinction) and fluorescent emission. For more application areas, see image 1 below.

e.g. Zebrafish embryos can be characterized by profiling the optical density (EXT) and fluorescence pattern along the axial length (TOF). See image 2 below for more details.


System configuration:

3 Lasers, 3 Fluorescence detectors (PMTs)

Large Particle (LP) Sampler: Sample-Input from multi-well plates, petri dishes, 50 ml or 750 ml stirred sample cups

Sorted samples: can be dispensed as single objects or bulk sorts into 24-, 48-, 96-, and 384-multiwell plates and 15 or 50ml  tubes.

Flow cells: FOCA250 (Diameter: 250 µm), FOCA1000 (Diameter: 1000 µm)

 

Laser 1

405 nm Violet Laser, 50 mW

Detection

445nm/45 nm – BV421, Alexa Fluor 405, DAPI, Hoechst, Pacific Blue, BFP

Laser 2

488 nm Blue Laser, 50 mW

Detection

510nm/23 nm - FITC, GFP, YFP, CFP, Alexa Fluor 488
535nm/45 nm - mVenus

Laser 3

561 nm Yellow Green Laser, 50mW

Detection

586/20 nm - MKO
615/24 nm - PE-Texas Red, PI, mCherry

 

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