BioOptics - Light Microscopy
"Learn to live with Uncertainty"
The BioOptics facility is dedicated to providing state-of-the art light microscopy equipment to MFPL researchers.The facility has been centralized in rooms of the VBC5-building. Currently, three laser scanning confocal microscopes, two spinning disc units, a live-imaging station equipped for TIRF with full environmental control and microfluidics, an epifluorescence deconvolution microscope, a high-content imaging station and a microdissection-laser ablation instrument are available. The facility management provides professional training and assists in experimental planning, technical setup, troubleshooting and image analysis.
July 2017: Publication in NAR with facility contribution
Tanja Kaufmann, Irina Grishkovskaya, Anton A. Polyansky, Sebastian Kostrhon, Eva Kukolj, Karin M. Olek, Sebastien Herbert, Etienne Beltzung, Karl Mechtler, Thomas Peterbauer, Josef Gotzmann, Lijuan Zhang, Markus Hartl, Bojan Zagrovic, Kareem Elsayad, Kristina Djinovic-Carugo and Dea Slade: “A novel non-canonical PIP-box mediates PARG interaction with PCNA.“ Nucleic Acids Research (July 2017). DOI: https://doi.org/10.1093/nar/gkx604
June 2017: New image analysis workstation
Our deconvolution workstation running the Huygens package has been replaced by a blazing fast high-end computer (2x Xeon E5 2680 CPU, 3x NVIDIA Geforce GTX1080 GPU with 3x 8 GB, 256 GB RAM). Enjoy!
January 2017: High Content Microscope (HCM) installed and ready for use
We have successfully re-configured an existing microscope unit to a High-Content Imaging station. The unit has been completely re-programmed and is driven by non-commercial acquisition software, which also allows us flexibility in integrating optical equipment. In principle the design (allowing phase contrast) is focused at fluorescence imaging of multi-well plates (plastic and glass-bottom) of any format using a range of long-distance dry objectives. More comprehensively the system may be used for other individualized approaches.
Location and Rooms
VBC5 - Structural and Computational Biology Dept., Level E1
- Office: room 1.618 (tel. ext. 61672)
- Microscopes 1: room 1.223 (tel. ext. 61678)
- Microscopes 2: room 1.320 (tel. ext. 61677)
- Microscopes 3: room 1.318 (tel. ext. 61679)
- Tissue Culture: room 1.219
- Image Processing workstations: MFPL main building, 6th floor, room 6.508.
E-mail: lightmicroscopy [AT] mfpl.ac [DOT] at
Josef Gotzmann: +43-664-8001635200
Thomas Peterbauer: +43-664-60277-52878
Available Equipment (room #)
- Inverse confocal Zeiss LSM 710 with Airyscan detector (1.223)
- Inverse confocal Zeiss LSM 700 (1.223)
- Upright confocal Zeiss LSM 510 META (1.320)
- Inverse Visitron Spinning Disk unit with FRAP (1.223)
- Inverse Live Spinning Disk/Nanodissection unit (1.318)
- Inverse Deltavision epifluorescence microscope (1.320)
- Inverse live imaging unit Olympus cellSens /TIRF (1.223)
- Upright microdissection unit Leica LMD6500 (1.320)
- Inverse High Content Microscope unit (HCM) (1.223)
- Two image processing workstations (room 6.508, MFPL main building) providing stand-alone microscope software (Olympus cellSens, ZEN 2012, Visiview) and professional software packages for deconvolution and 3/4D image processing (SVI Huygens, softWoRx suite)
- Zeiss Stereomicroscope Stemi2000 (Transmitted/Incident Light)-room 1.320
- Leica Stereomicroscope S6E (Transmitted/Incident Light)-room 1.318
- BSL2 Tissue Culture Hood (room 1.219)
- BSL1 Tissue Culture (room 1.219, hood, microscope, incubator, freezer & fridge)
Potential trainees must
- provide an organized experimental strategy to discuss with the facility staff
- already have own samples for a specialized training session
- Fill in the Light Microscopy Training Application form and meet the facility staff (appointments via lightmicroscopy [AT] mfpl.ac [DOT] at) to discuss most forward strategies and find the proper microscope(s) to be trained on.*
- Attend the "Introductory Lecture Biooptics" including laser safety instructions.**
- Organize a training unit with the facility staff – training units will be split into "how to do" and "optimize my sample" sessions (on separate days).
- Fill in and sign the “Training Confirmation” form on usage of microscopes/user fee regulations (trainee and group leader) and bring it to your training session.
- Before you attend the sessions, please download our "General Administrative Rules" and read them thoroughly!
*Optional: facility personnel evaluates potential applicability with user specific samples, if selection of the proper microscope system remains unclear.
**1.) and 2.) may be switched.
Lectures always take place in the seminar rooms in VBC5, level E1 (Structural Biology Dept.). Lecture Dates are announced regularly. To register, login to the MFPL Intranet ⇒ "Booking System" ⇒ ”Lecture and Training Registration".
User Fee Strategy
MFPL users can access current prices via the intranet ("Booking System" ⇒ "Facility Infos"). External users in any case need to contact the head of facility, Josef Gotzmann, to get informed on conditions to use MFPL's Biooptics instrumentation.
In House Microscopes
Six microscope systems (one in each floor + histology + teaching microscope) in the MFPL main building have been upgraded to state-of-the-art technology. They are fully motorized, host high-end CCD cameras and are fully equipped for fluorescence/brightfield imaging. Irmgard Fischer is the responsible person for these microscopes: she helps in training and troubleshooting and maintenance/service.
Irmgard Fischer; Facility Technician; Rooms 5.528/5.530; Tel. ext. 52866; Irmgard.fischer [AT] univie.ac [DOT] at
- 2nd floor (room 2305) - Axiovert 200M
- 3rd floor (room 3517) - Axio Observer Z1
- 4th floor (room 4407) - Axio Imager Z2
- 5th floor (room 5423) - Axio Imager M2
- Histology (room 4518) - Axio Imager M1
- Teaching (room 5519) - Axio Observer Z1