News and Events
STEDYCON XY Superresolution System Installed
(April 20, 2018) We are happy to announce that our portfolio of high-end systems has grown by the STED superresolution microscope STEDYCON. We have started an initial training phase and will be fully operational by beginning of May.
Spinning Disk Upgrade: EM-CCD
(March 8, 2018) Our spinning disc microscope has been upgraded with a sensitive EM-CCD camera (Hamamatsu ImagEM X2). The acquisition software Visiview was upgraded to version 4.x and now allows Phython based scripting.
User Meeting 2018
(March 9, 2018) The 2018 User Meeting will cover information on upcoming workshops, a replique on the face-to-face initiative, changes in the room situation and presentations on all upcoming investments. As usual, the user meeting provides a forum for facility users to discuss general and specific demand and wishes. The meeting will take place in MFPL main building, 6th floor, Seminar Room 1 (2.00-4.00 pm). Download the presentation.
Deltavision ULTRA Workshop
(March 9, 2018) We have organized a workshop with GE to showcase their newest fluorescent microscope system, the Deltavision "Ultra". After an introductory talk at March 20 (9.30am), we will extensively test the microscope setup with our own samples. Please find the invitation attached.
Publication in EMBO Journal with facility contribution
(Jan. 2018) Gabriele Zaffagnini, Adriana Savova, Alberto Danieli, Julia Romanov, Shirley Tremel, Michael Ebner, Thomas Peterbauer, Martin Sztacho, Riccardo Trapannone, Abul K Tarafder, Carsten Sachse and Sascha Martens : “p62 filaments capture and present ubiquinated cargos for autophagy .“ EMBO J, DOI:http://onlinelibrary.wiley.com/doi/10.15252/embj.201798308/abstract
HIVE Acquisition server operational
(Oct. 23, 2017) Our HIVE acquisition server starts routine operation with connections to our confocals, spinning disks, and the Olympus cellSens Live/TIRF station.
T-control unit "Cherry-Temp" available
(Aug. 2017) Thanks to a Vienna Doctoral School stipend awarded to Triin Laos (Dammermann group) and support from Alex Dammermann and Sascha Martens, a versatile temperature-switch control system could be purchased. This unit allows cooling and fast temperature switches. It is currently installed at the Spinning Disc microscope.
Publication in NAR with facility contribution
(July 2017) Tanja Kaufmann, Irina Grishkovskaya, Anton A. Polyansky, Sebastian Kostrhon, Eva Kukolj, Karin M. Olek, Sebastien Herbert, Etienne Beltzung, Karl Mechtler, Thomas Peterbauer, Josef Gotzmann, Lijuan Zhang, Markus Hartl, Bojan Zagrovic, Kareem Elsayad, Kristina Djinovic-Carugo and Dea Slade: “A novel non-canonical PIP-box mediates PARG interaction with PCNA.“ Nucleic Acids Research, DOI: https://doi.org/10.1093/nar/gkx604
New image analysis workstation
(June 2017) Our deconvolution workstation running the Huygens package has been replaced by a blazing fast high-end computer (2x Xeon E5 2680 CPU, 3x NVIDIA Geforce GTX1080 GPU with 3x 8 GB, 256 GB RAM). Enjoy!
High Content Microscope (HCM) installed
(Jan. 2017) We have successfully re-configured an existing microscope unit to a High-Content Imaging station. The unit has been completely re-programmed and is driven by non-commercial acquisition software, which also allows us flexibility in integrating optical equipment. In principle the design (allowing phase contrast) is focused at fluorescence imaging of multi-well plates (plastic and glass-bottom) of any format using a range of long-distance dry objectives. More comprehensively the system may be used for other individualized approaches.
TIRF upgrade installed at Olympus cellSens
(Dec. 19, 2016) We have now upgraded our live-widefield imaging unit, Olympus cellSens, with a TIRF-module. We offer three independent laser lines (405, 488, 561 nm), a 100x TIRF objective (NA 1.49) and a sensitive EM-CCD camera (16 x 16µm, 512 x 512 pixel). The 405 nm laser can also be used for photomanipulation.
Atomic Force Microscopy Workshop
(Feb. 9, 2017) We have organized a workshop on Atomic Force Microscopy (AFM) with JPK instruments, showcasing their newest developments (NanoWizard, www.jpk.com/nanowizard-r-4-overview.856.en.html) for addressing biological questions using AFM.The workshop will be a mix of lectures and extended hands-on sessions.
Tissue Culture Facility moved to room 1.219
(Aug. 12, 2016) The tissue culture equipment (2 incubators, two hoods), which can be co-used by facility users, has become moved from room 1.613 to room 1.219.
Server solution "HIVE" installed
(July 22, 2016) A modular server solution, “HIVE”, for image handling, storage, processing and analysis is now installed. Currently we connected the HIVE via an Intranet solution to the microscopes – a 10 Gbit/s solution is currently under construction. Initially the HIVE will be used as a fast data transfer solution - a kind of intermediate data distributor. We are working on semi-automated transfer and fully automated image processing/analysis solutions, which are to be be implemented soon.
"In House Microscopes" inserted in Intranet Booking System
(July 8, 2016) Our "In House Microscopes", which are administered by Irmgard Fischer, are now all available for booking in our established Intranet reservation system. The "In House Microscopes" can easily be found under the identically named tag. Only the histo-microscope remained with the Histo-Facility tag.
New z-piezo stage installed at LSM 710-Airy
(July 7, 2016) The scanning stage at the confocal LSM 710-Airy has become replaced by a modernized z-piezo stage with respective inserts. The z-piezo drive is also compatible with Airy-scanning using the respective Airy-insert. Full flexibility in terms of fast and precise z-scanning is therefore established at the LSM 710-Airy.
(Sept. 30, 2015) Our old Olympus cell^R live imaging station has been upgraded to a cellSens unit. The system features a fast and sensitive sCMOS camera, Sedat setup for fast acquisition, full environmental control and a microfluidic device for experiments on stage.
Live spinning disk equipped for widefield and split imaging
(July 30, 2015) Our spinning disk unit for live imaging, FRAP, and nanodissection has been upgraded for widefield fluorescence imaging including another sCMOS camera. The widefield configuration is a SEDAT-setup, allowing combinations of all standard fluorophores. An OptoSplit device has additionally been installed for simultaneous two-colour imaging (e.g. ratio or FRET analyses).
User Meeting 2015
(Sept. 30, 2015) The 2015 User Meeting will cover presentations on theory and applicability of new equipment (Airy Scan detector upgrade, Cell Sense live imaging unit, high-content plate imaging system) and provides a forum for facility users to discuss general and specific demand and wishes.The meeting will take place in MFPL main building, 6th floor, Seminar Room 1 (2.30-3.30 pm). Download the presentation.
Bioluminescence imaging workshop
(Sept. 22-23, 2015) During this workshop (see invitation and programme) we will demo the Olympus LV200 microscope system, dedicated for bioluminescence. Besides a theoretical introduction we offer extensive hands-on possibilities.
New spinning disk system
(October 27, 2014) Our new spinning disk unit for live imaging, FRAP, and nanodissection is up an running!
New confocal microscope Zeiss LSM 700 up and running
(July 24, 2014) The old LSM 510 scan head has retired after 16 years and was replaced by an LSM 700.
Image processing workstations moved
(July 10, 2014) Our workstations found a new home in the MFPL main building (6th floor, room 6.508).
User meeting June 10, 2014 (14:30, MFPL SR1, 6th floor)
(May 26, 2014) Technical features and applicability of a new confocal and a new spinning disc unit will be presented. All users of the facility are cordially invited.
(Sept. 27, 2013) As of October 1st, new training routines with individualized training sessions are implemented.
(Aug. 30, 2013) September 24th and 25th 2013, building VBC5, Level E1.
Two companies will showcase their commercial products making use of innovative techniques to image live cells.
Welcome to our new facility technician!
(July 18, 2013) This week, our new facility technician, Thomas Peterbauer, started working. His major duties will cover training support and help with image processing. His expertise will be a valuable help and will comprehend existing knowledge. His contact details: Tel-ext.: 61676; e-mail: thomas.peterbauer [AT] univie.ac [DOT] at mobile: 0664-60277-52878;
SIM and FLIM systems installed and operational soon:
(April 11, 2013) The two new high-end microscope systems for use of SIM- and FLIM-/FCS-technologies, respectively, have now been successfully installed under the auspices of the CSF. More details on equipment and usage conditions can be found at the CSF-Advanced Microscopy homepage: http://www.csf.ac.at/am/
CSF microscopes to be installed soon.
(Feb. 7, 2013) Under the auspices of the CSF two new microscope systems will be available soon.
First, a Picoquant MT-200 system, designed for FLIM(-FRET), FCS, FCCS measurements will be installed beginning of April. Secondly, an OMX-Blaze structured illumination super-resolution system will arrive in March. Details on locations, training, usage to come.
Technical and applicative information at: http://www.picoquant.com/products/microtime200/microtime200.htm and http://www.api.com/deltavision-omx.asp
We are looking for a motivated person to support our team!
(Feb. 25, 2013) He/she shall fill a vacant University technician position at the MFPL biooptics facility.
Details and applications at the University of Vienna jobcenter (http://jobcenter.univie.ac.at/fuer-bewerberinnen/)and advertisement # 3803 (Kennzahl 3803):
Spinning disk microscope
(May 12, 2012) Our latest add-on is a laser-based spinning disk confocal microscope system including a 405 nm FRAP-option from Visitron.
(Jan. 23, 2012) Confocal training routine, covering instruction for all 3 confocal microscope systems, established.
(Oct. 10, 2012) The new confocal laser scanning microscope ZEISS LSM 710 is installed and ready to use. Mandatory laser safety instructions to be held in January/February 2012.
Have a look at our Vienna-Bioimaging wiki at