Biochemistry & Biophysics | Genetics, Epigenetics & Gene Regulation
RNA Aptamers and RNA Chaperones
renee.schroeder [AT] univie.ac [DOT] at
Dr. Bohr-Gasse 9, 1030 Vienna | Room: 5.113
RNA is at the center of all steps of gene expression. Cells can be defined by their transcriptomes, not by their genomes. We are interested in discovering many regulatory elements that are part of the RNA regulon and in identifying their interacting partners and their targets. To achieve this goal we adapted the classical SELEX procedure to be used in combination with genome sequences and deep sequencing. Genomic systematic...more
RNA is at the center of all steps of gene expression. Cells can be defined by their transcriptomes, not by their genomes. We are interested in discovering many regulatory elements that are part of the RNA regulon and in identifying their interacting partners and their targets. To achieve this goal we adapted the classical SELEX procedure to be used in combination with genome sequences and deep sequencing. Genomic systematic evolution of ligands by exponential enrichment (SELEX) allows the isolation of protein binding RNAs independently of computational predictions and expression conditions. We used genomic SELEX with an E. coli library to isolate RNA aptamers against RNA polymerase and the regulator protein Hfq. We further selected RNA polymerase II binding aptamers from the yeast and human genomes. These experiments delivered thousands of genomic RNA aptamers that regulate gene expression. We are currently analyzing the mode of action of these aptamers.
Another focus in our laboratory deals with proteins that promote RNA folding: RNA chaperones. As model examples we are analyzing the mode of action of the E. coli protein StpA and the HIV-1 Tat peptide. While StpA promotes RNA annealing and strand exchange, HIV-1 Tat only promotes RNA annealing. Using biochemical and biophysical methods (NMR) we study the structural dynamics of both RNA and protein.
Matylla-Kulinska, Katarzyna; Boots, Jennifer L; Zimmermann, Bob; Schroeder, Renée (2012). Finding aptamers and small ribozymes in unexpected places. Wiley Interdiscip Rev RNA;1(3):73-91. PMID: 21853532
Doetsch, Martina; Schroeder, Renée; Fürtig, Boris (2011). Transient RNA-protein interactions in RNA folding. FEBS J;278(10):1634-42. PMID: 21410645
Doetsch, Martina; Fürtig, Boris; Gstrein, Thomas; Stampfl, Sabine; Schroeder, Renée (2011). The RNA annealing mechanism of the HIV-1 Tat peptide: conversion of the RNA into an annealing-competent conformation. NUCLEIC ACIDS RES;39(10):4405-18. PMID: 21297117
Doctoral Program "RNA Biology"
The Group Schroeder participates in the special Doctoral Program "RNA Biology" reviewed and funded by the Austrian Research Fund FWF.
SFB "RNA-Reg - RNA regulation of the transcriptome"
Renée Schroeder is speaker of the Special Research Area (SFB) "RNA-Reg - RNA regulation of the transcriptome" funded by the Austrian Science Fund FWF. SFB's are peer-reviewed, highly interactive research networks, established to foster long-term, interdisciplinary co-operation of local research groups working on the frontiers of their thematic areas.
Eduard Buchner Prize 2011
German Society for Biochemistry and Molecular Biology (GBM) > view news message
Member of the Austrian Council for Research and Technology
for the function period 2010-2016
awarded by the Austrian Science Fund FWF on behalf of the Ministry for Science and Research.
Science Book of the Year 2012
Read more about Renée Schroeder's prize-winning book here.
Doris Chen - doris.chen(at)univie.ac.at
Katharina Semrad - katharina.semrad(at)univie.ac.at
Sabine Stampfl - sabine.stampfl(at)univie.ac.at
Oliver Mayer - olivengeist(at)gmx.at
Lukas (Rajkowitsch) Paul - l.p(at)gmx.net
Nisha Joseph - nishjos(at)gmail.com
Stefan Ameres - stefan.ameres(at)umassmed.edu
Frederike von Pelchrzim - rikevp(at)aol.com
Kira Jelencsic - kira.jelencsics(at)univie.ac.at
Ursula Schöberl (née Schauer) - ursula.schoeberl(at)imba.oeaw.ac.at
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Nikolai Windbichler - nikolai.windbichler(at)imperial.ac.uk
Petra Böck-Taferner - petra.boeck-taferner(at)gmx.at
Rupert Grossberger - r.grossberger(at)sanochemia.at
Michael Werner - michaelwerner(at)gmx.at
Albert Weixlbaumer - trebla(at)gmx.at
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